| Group | Club/Team Name | Team ID | Head Coach |
| A1 | z-Elite Soccer EBA 08 Maroon Lincoln |
02075-001SB12-0989 | Thomas Burkey |
| A2 | z-Developmental Players Academy DPA Bafana 08 |
0N076-001SB12-0004 | Kyle Deremer |
| A3 | Papillion Soccer Club PSC 2008B Maroon |
02016-001SB12-0019 | Jentry Schram |
| A4 | Nebraska FC NFC Red 2008 |
03062-001BB12-0115 | Jake Grimes |
| A5 | z-Omaha Nitro Futbol Club ONFC Omaha United SC - BU12 |
03083-001PB12-0082 | Daniel Vasquez Gutierrez |
| A6 | Sporting Nebraska FC SOFC B SILVER 08 |
03036-001GB12-0104 | Jonathan Iberg |
| A7 | Dreamers FC DFC Midwest Premier Academy Blue Omaha 08B |
03134-001SB12-0031 | David Hawkins |
| A8 | Elkhorn Athletic Association ESC Sparta 2008B |
03004-001NB12-0936 | Haley Shelton |